Uw huidige browser heeft updates nodig. Zolang u niet update zullen bepaalde functionaliteiten op de website niet beschikbaar zijn.
Let op: het geselecteerde rooster heeft overlappende bijeenkomsten.
Volgens onze gegevens heb je nog geen vakken behaald.
Je planning is nog niet opgeslagen
Let op! Uw planning heeft vakken in dezelfde periode met overlappend timeslot
Cell Biology and Cellular Signalling Lab (2024/2025: periode 4)
Cursusdoel
.In this lab course you will:
1. Get acquainted with a number of basic techniques used in molecular (cell) biology.
Principle and practice of: gel electrophoresis (SDS-PAGE), immunoblotting (Western blotting, immunofluorescence, plasmid isolation and transfection of cultured cells.
2. Learn some details and background of signal transduction
3. Learn some details about antibodies
4. Learn how to write/keep up a lab journal
5. Learn how to work safely in the lab
6. Develop a research mentality
1. Get acquainted with a number of basic techniques used in molecular (cell) biology.
Principle and practice of: gel electrophoresis (SDS-PAGE), immunoblotting (Western blotting, immunofluorescence, plasmid isolation and transfection of cultured cells.
2. Learn some details and background of signal transduction
3. Learn some details about antibodies
4. Learn how to write/keep up a lab journal
5. Learn how to work safely in the lab
6. Develop a research mentality
Vakinhoudelijk
This course is an introduction to signal transduction pathways in mammalian cells, more specifically growth factor signalling and the response of cells upon activation of this signalling. Clearly these mechanisms are important in both (cellular) physiology and pathology and are for instance studied to understand the mechanisms of uncontrolled cell growth (cancer). For this course cultured eukaryotic cells are used as a model system. Molecular biology techniques such as Gel electrophoresis (PAGE), Western blotting, Immuno-precipitation, and Immuno-fluorescence are used to investigate signal transduction pathways. The theory of the techniques used in this course is addressed and a lot of attention is given to designing the experimental set-up, performing the experiments and analysing the data. Throughout the course, students keep up a lab journal as this is the basis for conduction good research.
Lab course attendance is 100% mandatory.
Werkvormen
lab course
Toetsing
Practical lab work and lab journal
Verplicht | Weging 100% | ECTS 2,5
Ingangseisen en voorkennis
Ingangseisen
Er moet voldaan zijn aan de cursus:
Voorkennis
Er is geen informatie over benodigde voorkennis bekend.
Voertalen
- Engels
Competenties
-
Academisch schrijven
-
Luisteren
-
Onderzoeksvaardigheden
-
Samenwerken
Cursusmomenten
Gerelateerde studies
Tentamens
Er is geen tentamenrooster beschikbaar voor deze cursus
Verplicht materiaal
Er is geen informatie over de verplichte literatuur bekend
Aanbevolen materiaal
-
BOEKCampbell Biology Pearson eText, Global Edition, 12/E. Campbell Neil A, Urry Lisa A, Cain Michael L, Wasserman Steven A, Minorsky Peter V, Orr Rebecca B. 2021. Pearson. ISBN/EAN 9781292341637,
Opmerkingen
Registration for lab courses closes approx. 3 months prior to start. 3 modules to be taken in 2 consecutive terms
Coördinator
dr. G.S.A.T. van Rossum | G.S.A.T.vanRossum@uu.nl |
Docenten
dr. G.S.A.T. van Rossum | G.S.A.T.vanRossum@uu.nl |
Inschrijving
Let op: deze cursus is niet toegankelijk voor studenten van andere faculteiten, bijvakkers mogen zich dus niet inschrijven.
Naar OSIRIS-inschrijvingen
Permanente link naar de cursuspagina
Laat in de Cursus-Catalogus zien