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Microscopy and Image Analysis
Cursusdoel
At the end of this course you will:
- know the basic physical principles underlying light and electron microscopy
- understand the workings of modern light and electron microscopes
- understand the principles of specialized light microscopy techniques, such as confocal microscopy, TIRF microscopy, Super-resolution microscopy
- know how to choose between different labeling strategies to generate fluorescence contrast
- be familiar with the different applications of light and electron microscopy
- know how to analyze microscopy images and generate paper figures
Vaardigheden - Skills
- Hands-on experience with acquiring fixed and live imaging on an upright microscope
- Hands-on experience with performing image analysis
- Analyzing primary literature and presenting it.
- Critical thinking
- Use vector based software for figure assembly
Vakinhoudelijk
The level 2 course B-B2MGOT14 or comparable knowledge is a prerequisite. The course De Cel (B-B2CEL09) is recommended. As of 22-23 the B-B3MIA22 course replaces the B-B3LEM18 course for the biology bachelor. It is not possible to follow both.
Studiepad
This course fits within the study path Cell Biology and Developmental Biology. It provides a good preparation for the Master program Molecular and Cellular Life Sciences.
Language
This course is partially English taught (depending on the teacher). All course material is English
Inhoud - Content
The aim of this course is to provide the students with a deep understanding of modern microscopy techniques in biology, with an emphasis on fluorescent microscopy techniques and the applications in cell biology.
The course will consist of lectures (HC) covering the theory accompanied by tutorials (WC). In additions, the course will provide practicals where the students will have the opportunity to get hands-on experience with fluorescent microscopes.
Finally, the students will be taught how to analyze, quantify and represent microscopy images.
Werkvormen
Hoorcolleges
Werkcolleges
Practica
Presentatie
Zelfstudie
Schrijfopdracht
Toetsing - assessment
The final grade for the course will consist of:
Exam 50%
Report 30%
Presentation 20%
Each grade should be a 5 or higher to pass.
In addition, attendance of and active participation in the Practicals is obligatory and will be registered (inspanningsverplichting). You cannot pass the course if you were absence without prior approval.
Studiemateriaal
- Murphy and Davidson, Fundamental of Light Microscopy and Electronic Imaging.
http://onlinelibrary.wiley.com/book/10.1002/9781118382905
- Manual practicals
- Manual image analysis
- Lecture slides
Werkvormen
Inzage
Practicum
Werkcollege
Toetsing
Eindresultaat
Verplicht | Weging 100% | ECTS 7,5
Ingangseisen en voorkennis
Ingangseisen
Je moet inschreven staan voor een van de volgende opleidingen:
- Biologie
- Scheikunde
Voorkennis
The level 2 course B-B2MGOT14 or comparable knowledge is a prerequisite. The course De Cel (B-B2CEL09) is recommended. As of 22-23 the B-B3MIA22 course replaces the B-B3LEM18 course for the biology bachelor. It is not possible to follow both.
Voertalen
- Engels
Cursusmomenten
Gerelateerde studies
Tentamens
Er is geen tentamenrooster beschikbaar voor deze cursus
Verplicht materiaal
Materiaal | Omschrijving |
---|---|
BOEK | Murphy and Davidson, Fundamental of Light Microscopy and Electronic Imaging. Online available through the UU library http://onlinelibrary.wiley.com/book/10.1002/9781118382905 |
DICTAAT | ─ Manual practicals ─ Manual image analysis ─ Lecture slides |
Aanbevolen materiaal
Er is geen informatie over de aanbevolen literatuur bekend
Coördinator
dr. M. Harterink | M.Harterink@uu.nl |
Docenten
Inschrijving
Inschrijving
Van maandag 18 september 2023 tot en met vrijdag 29 september 2023
Na-inschrijving
Van maandag 23 oktober 2023 tot en met dinsdag 24 oktober 2023
Inschrijving niet geopend
Permanente link naar de cursuspagina
Laat in de Cursus-Catalogus zien